Dosage de sucre : quelle colonne ?

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

7 posts Page 1 of 1
Bonjour,
Je suis étudiante en chimie analytique et je débute mon stage.
Comme indiqué dans le titre, j'aimerais savoir quelle est le meilleur choix de colonne pour le dosage de sucre.
Merci d'avance pour vos futures réponses. :P
Manon
Hi,
I'm a student in analytical chemistry, and I'm starting an internship.
As written in the subject, I would like to know which is the best column to analyse sugar.
Thank you in advance :P ,
Manon
Good Morning,

Will you use HPLC and refractive index detection or IC and Integrated Pulsed Amperometric Detection?
MattM
I will use HPLC ! :)
Bonjour Manon,

First of all you need to state which sugars you want to separate. Please check out the websites from column manufacturers for sugar analysis and look for the most suitable application. Check out Shodex, Phenomenex, Waters, MAcherey & Nagel, Agilent, Knauer. Ionexchangers are often used or NH2 Amino columns. What is the best depends mostly on the matrix. Good luck
Gerhard Kratz, Kratz_Gerhard@web.de
Hello again,

Well, it's been a while, I recall that one standard, reverse phase method for sugars uses an aminopropyl phase; here's an example application in this paper.

http://www.academia.edu/4850487/HPLC_an ... s_in_foods

Please take a look and see what you think. My thanks! Note, detection was by Refractive Index, if you have this detector.
MattM
Pour le moment, j'aime la colonne Shodex NGP-50. Le revêtement des colonnes d'amino-silice tend à être instable. Étant polymèrique, la colonne Shodex évite ce problème. De plus, les groupes amine sont tertiaires, excluant la (légère) possibilité de formation de base de Schiff avec des sucres réducteurs.
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