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analytical method for the quantitative analysis of Emodin.

Posted: Mon Jan 22, 2018 3:26 pm
by restandrelax
Need advice on whether gc or hplc should be used. And help on sample preparation, and mobile phase to be used.
The product is in a oil dosage form so how do i go about it?

Re: analytical method for the quantitative analysis of Emodin.

Posted: Mon Jan 22, 2018 6:21 pm
by HPLC chemist
GC is only useful if the molecule is volatile and detectable (FID, TCD, ECD, MS).

HPLC is only useful if the molecule is soluble and detectable (RI, UV, DAD/PDA, PAD, ELSD, CAD, MS).

The other questions are what molecular target, what sample matrix, and what concentration (these will determine the appropriate instrument).

Re: analytical method for the quantitative analysis of Emodin.

Posted: Mon Jan 22, 2018 6:27 pm
by HPLC chemist
HPLC equipped with a DAD/PDA! Why reinvent the wheel.

http://pubs.acs.org/doi/abs/10.1021/jf304804c

Re: analytical method for the quantitative analysis of Emodin.

Posted: Mon Jan 22, 2018 7:33 pm
by HPLC chemist
Mind you I would try a 5 cm C18 column 1st. Proper baseline separation depends on the sample matrix, but I doubt your dietary supplement will be more challenging than a plant!

Re: analytical method for the quantitative analysis of Emodin.

Posted: Sun Feb 11, 2018 11:21 am
by mattmullaney
Hello,

I agree with HPLC Chemist...for GC, derivatization is required for Emodin. Why bother if you do not have to?