HPLC of polyphenols in aqueous Tween 80

[jennivee.chua]

I wish to quantify the catechin in my plant extracts with 1%-7.5% Tween 80 using a C18 column. I have seen studies that analyzed cloud point extracts in HPLC but won't the samples bubble and cause problems? Has anyone tried analyzing polyphenols in polysorbate solutions? I was thinking maybe diluting it 10-100x (to fit within calibration curve as well) but will this damage the column?

[itspip]

Are you thinking about micellar chromatography? Is you extract containing Tween, or is the mobile phase for the LC containing Tween?

This paper might be a good place to start; https://www.mdpi.com/2297-8739/9/3/61