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internal standard resonce ratio
Posted: Wed Feb 17, 2010 4:44 pm
by nageshiict
I am doing bio analytical analysis by using internal standard.but i am getting more intenced peak for internal standard than analytes. For how much responce ratio, i have to use for internal standard analysis.please suggest me.
Posted: Wed Feb 17, 2010 5:58 pm
by HW Mueller
HPLC is a method which has to be calibrated.
Posted: Wed Feb 17, 2010 5:59 pm
by JGK
Aim for an IS concentration that will produce a response similar to that seen at the mid point of your analyte calibration range
Posted: Fri Feb 19, 2010 5:09 pm
by nageshiict
Mr JGK
thank u for good suggetion