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Setting retention time windows for EPA 300.0

Posted: Fri Feb 27, 2026 2:35 pm
by Jofichrom
I am new and running EPA 300.0 on an ICS-6000 and I'm trying to make sure the processing methods are following the DEP guidelines. After calibration, the current retention times are set in the component table tab. But as far as the retention time windows, how is that set? The method states "3 times the standard deviation of the retention time" so am I adjusting this percentage each time as well to that result? Or is that formula for something else?
Forgive my inexperience but I can't seem to find an answer in the software or elsewhere.

Re: Setting retention time windows for EPA 300.0

Posted: Fri Feb 27, 2026 8:35 pm
by DR
Please include a mention of the data system you're using. ICS 6000 speaks to several CDS platforms...

My suspicion is that you make 3 or 4 injections, calculate a mean value and standard deviation for each peak of interest and set your retention window to be approximately RT±3sd, and then you can forget it.

Depending on your CDS, there may be a convenient box to check to accomplish this in your processing method.