Chromatography Forum

I'm looking for information regarding the typical range of peak capacities that can be obtained with state-of-the-art commercial LC. If it helps, we can narrow the scope of this question by assuming small molecules and/or peptides and gradient elution, reversed-phase UHPLC/UPLC. I understand that this will depend on numerous factors and therefore the answer would give a relatively broad range of values. From my experience, a peak capacity between 100-300 seems about right. I’m having trouble locating a reference (recent journal article or book) that would contain this information. Any information on this topic would be helpful but a scientific reference would be best. Thanks in advance.

Here are a few that might help:

Stephen A.C. Wren, Peak capacity in gradient ultra performance liquid chromatography (UPLC), Journal of Pharmaceutical and Biomedical Analysis, Volume 38, Issue 2, 15 June 2005, Pages 337-343, ISSN 0731-7085, DOI: 10.1016/j.jpba.2004.12.028

Francois Lestremau, Di Wu, Roman Szucs, Evaluation of 1.0 mm i.d. column performances on ultra high pressure liquid chromatography instrumentation, Journal of Chromatography A, In Press, Corrected Proof, Available online 31 May 2010, ISSN 0021-9673, DOI: 10.1016/j.chroma.2010.05.044.

Davy Guillarme, Elia Grata, Gaetan Glauser, Jean-Luc Wolfender, Jean-Luc Veuthey, Serge Rudaz, Some solutions to obtain very efficient separations in isocratic and gradient modes using small particles size and ultra-high pressure, Journal of Chromatography A, Volume 1216, Issue 15, 10 April 2009, Pages 3232-3243, ISSN 0021-9673, DOI: 10.1016/j.chroma.2009.02.032.
(http://www.sciencedirect.com/science/ar ... ef40f0f0c3)

And a good explanation/discussion in Snyder & Dolan's "High Performance Gradient Elution" book on pages 47-49. Here's a link to the book on Amazon: http://tinyurl.com/2dqdrgj

Thanks again. Your help has been appreciated.