Chromatography Forum

Hi,

I would appreciate any suggestion about where I can buy a reliable pre-packed DEAE anion exchange column for protein and DNA analysis. I need to use that in Perkin Elmer autosampler HPLC system so I have to be cautious about packing it by myself since I have not done that before.

Thankssss in advance

Zahra

Zahra:

PolyLC sells PolyWAX LP columns. While the functional group is not DEAE-, this material is still a weak anion-exchange (WAX) material, which is the relevant concern for your two applications. You should get a material with a pore diameter of either 1000- or 1500-Å (the 1500-Å material is particularly effective for PCR reaction product purification).

Thank you very much Andy. That was very useful.

Hi Zahra,

Here is a link of one DNA analysis we have done using our SAX(prepacked DEAE) column: http://www.sepax-tech.com/application_notes/PD1002.pdf; one newsletter on this topic as well: http://archive.constantcontact.com/fs00 ... 41547.html FYI

You can find more application on protein, peptide and Polysaccharides in the application tab here: http://www.sepax-tech.com/Proteomix.php

We also develop one good data using polymer based reverse phase column on DNA analysis, please feel free to contact me twu@sepax-tech.com if you are interested in.

Ting

Ting Wu:

A DEAE- functional group is a tertiary amine; it stands for "diethylaminoethyl-". A material with DEAE- groups is a weak anion-exchange material (WAX-type). Strong anion-exchange (SAX) materials have quaternary amine functional groups. The difference in performance (other than the more hydrophobic character of a SAX material) is that WAX materials lose their (+) charge in a continuum from pH 5 to pH 9.5, while SAX materials retain most of their charge as high as pH 10. Now, which kind of material does Sepax make?

Hi Zahra,

Here is a link of one DNA analysis we have done using our SAX(prepacked DEAE) column: http://www.sepax-tech.com/application_notes/PD1002.pdf; one newsletter on this topic as well: http://archive.constantcontact.com/fs00 ... 41547.html FYI

You can find more application on protein, peptide and Polysaccharides in the application tab here: http://www.sepax-tech.com/Proteomix.php

We also develop one good data using polymer based reverse phase column on DNA analysis, please feel free to contact me twu@sepax-tech.com if you are interested in.

Ting

Thank you Ting that was very helpful comments.

Ting Wu:

A DEAE- functional group is a tertiary amine; it stands for "diethylaminoethyl-". A material with DEAE- groups is a weak anion-exchange material (WAX-type). Strong anion-exchange (SAX) materials have quaternary amine functional groups. The difference in performance (other than the more hydrophobic character of a SAX material) is that WAX materials lose their (+) charge in a continuum from pH 5 to pH 9.5, while SAX materials retain most of their charge as high as pH 10. Now, which kind of material does Sepax make?

Hi Andy, we both offer DEAE and QAE column and did research on DNA analysis on both columns. Sorry I did not mention that in the previous communication. Thanks for pointing this out. The WAX data here: http://www.sepax-tech.com/application_notes/PD1003.pdf

Hi Zahra,

Here is a link of one DNA analysis we have done using our SAX(prepacked DEAE) column: http://www.sepax-tech.com/application_notes/PD1002.pdf; one newsletter on this topic as well: http://archive.constantcontact.com/fs00 ... 41547.html FYI

You can find more application on protein, peptide and Polysaccharides in the application tab here: http://www.sepax-tech.com/Proteomix.php

We also develop one good data using polymer based reverse phase column on DNA analysis, please feel free to contact me twu@sepax-tech.com if you are interested in.

Ting

Thank you Ting that was very helpful comments.

Hi Zahra, Sorry I made a mistake: DEAE is WAX column. We have a data on WAX column as well: http://www.sepax-tech.com/application_notes/PD1003.pdf Sorry for the confusion. You may find this data in the newsletter I mentioned previously too. Hope you will find a good solution on your test. Happy Friday!

Ting