Chromatography Forum

I can see from literatures that ACN and MeOH are the most common for metabolomics study, but no one mentioned the difference and perference.
Does anyone has some comparison articles for this purpose?
Thanks

I don't have an article, but for what it's worth:
Acetonitrile: lower viscosity so you can run it faster and the peaks tend to be better defined; slightly more eluting in RP chromatography; more expensive (and there was the great ACN shortage a while back).
MeOH: opposite to above, and a few things ionise in MeOH that don't ionise in ACN

Other differences: ACN turns to HCN inside you and poisons you; MeOH turns to formic acid inside you and poisons you, so both are fairly nasty for humans.

Thanks a lot for the helpful information.