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OPA/MPA derivatizatio stability for amino acids
Posted: Fri Feb 03, 2012 8:06 am
by dragosb
Hi,
I would like to know if the OPA/MPA derivatization results in stable products or is there something more I can do to avoid secondary derivatization products.
Please let me know about your opinion on this and what is your experience with the OPA/MPA derivatization.
Thank you very much
Re: OPA/MPA derivatizatio stability for amino acids
Posted: Fri Mar 16, 2012 12:03 pm
by PeterS
As far I know the products are not quite stable, so the best is to derivatize just before use in a time controlled situation. At least then there is a compareable situation between samples
Peter
Re: OPA/MPA derivatizatio stability for amino acids
Posted: Mon Mar 19, 2012 1:12 pm
by Kristof
As good as I know problem is not with secondary derivatization but with decrease of signal strenght in time due to decomposition of fluorescent product into non-fluorescent one.
With right type of autosampler you can run injector program that would perform OPA/MPA derivatization for you just before injection. I know Agilent 1100/1200 can do this.
For stable derivatization look at fluoresceine (less sensitive as good as I know) or AccQ Tag from Waters.
Re: OPA/MPA derivatizatio stability for amino acids
Posted: Mon Mar 19, 2012 8:26 pm
by JGK
As good as I know problem is not with secondary derivatization but with decrease of signal strenght in time due to decomposition of fluorescent product into non-fluorescent one.
With right type of autosampler you can run injector program that would perform OPA/MPA derivatization for you just before injection. I know Agilent 1100/1200 can do this.
For stable derivatization look at fluoresceine (less sensitive as good as I know) or AccQ Tag from Waters.
Check out the Pico tag from Waters if you have a standard HPLC. AccQ tag is for UPLC instruments.