Chromatography Forum

Hi,
We have an GPC Waters Breeze System(Isocratic HPLC pump 1515; 717 plus Autosampler, 2414 Refractive Index detector and 3 columns Styragel HR2xHR 0.5xHR0.5).In this moment we changed 2 valves on the detector:Solenoid valve and Relief valve, because it flows in the same time on the purje mode and on the analysis.Two days after this the GPC system it works, but after it begining again .What's happening? Please help me.
Thank you,
Ileana

hello,

It is possible that you have too much pressure in the system. In this case, the liquid flows by purge and relief valve at the same time.
Please check if your waste tubing or your purge out tubing are not plugged.
Maybe the internal diameter of these tubes are to small. Try to work without tubing just for test my theory.

I hope you will find one solution quickly,

Pierre

Thank you for your answer.You have right.We changed the wasting tube and the system is ok.
Ileana