Questions about LC-Ms buffers
Posted: Sat Nov 24, 2012 8:13 pm
Hello Everybody,
I have two short question about LC-MS buffers.
1. What is the maxiumum concentration of NH4Acetate or formiate that you use in the eluent? And why? (I have never used more than 20 mM)
2. Why can buffers improve the peak shape some of the compounds?
Many thanks in advance,
Zsolti
I have two short question about LC-MS buffers.
1. What is the maxiumum concentration of NH4Acetate or formiate that you use in the eluent? And why? (I have never used more than 20 mM)
2. Why can buffers improve the peak shape some of the compounds?
Many thanks in advance,
Zsolti