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front tailing
Posted: Tue Feb 05, 2013 5:17 pm
by push
Which factors can cause a front tailing in HPLC?
Re: front tailing
Posted: Tue Feb 05, 2013 5:57 pm
by Vlad Orlovsky
for some basic compounds it can be overloading of residual silanols at certain pH
Re: front tailing
Posted: Tue Feb 05, 2013 7:19 pm
by push
Thnks Vlad,
But the analyte does not contain nor basic neither acid groups.
Re: front tailing
Posted: Tue Feb 05, 2013 9:05 pm
by Consumer Products Guy
Inject less microliters, see if it gets better.
Re: front tailing
Posted: Tue Feb 05, 2013 10:03 pm
by Vlad Orlovsky
reasons:
overloading
poorly packed column (with fronting to help peaks with tailing)
diluent effect
solubility effect
tautomerism of compound (not defined "batman peak" for example)
Re: front tailing
Posted: Wed Feb 06, 2013 8:57 pm
by tom jupille
There is a chalk-talk that covers that:
viewtopic.php?f=31&t=18426
Fronting is discussed near the end (about 8 minutes in), but it's best to watch it all to put fronting into perspective.