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Agilent 1260
Posted: Mon Feb 23, 2015 10:22 am
by Besma2
WANT TO SCANNING A CAFFEINE SOLUTION WITH HPLC Agilent 1260 FOR CHECKING THE WAVE LENGTH, CAN YOU HELP ME PLEASE? HOW DO I PROGRAM MY METHOD (PART DETECTOR)
THANKS
Re: Agilent 1260
Posted: Mon Feb 23, 2015 9:42 pm
by AnalyticalChem
Caffeine absorbs in the spectral range between 244 nm to 300 nm
in dichloromethane and water with λ max at 276 nm and 272.8 nm respectively, 250 nm to 300 nm at
λ max of 276 nm in chloroform and 258 nm to 300 nm at λ max of 274.4nm in ethyl acetate.
Re: Agilent 1260
Posted: Tue Feb 24, 2015 7:02 am
by kubowicz.tomasz
Hello
DAD: inject caffeine and if you have spectra option installed you can collect full spectrum
VWD: fill cell with caffeine and take sample scan (you can use chemstation or hand controller)
Good luck
Tomasz Kubowicz
Re: Agilent 1260
Posted: Thu Feb 26, 2015 3:21 pm
by Besma2
Hello
Tomasz Kubowicz
thank you very much
I want to use ChemStation , you can please describe me the steps to create the scanning method and how to recover my results .
eg for the detector I have to select what
and once in the offline I check how the spectrum
thank you again