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Best buffers to use at alkaline pH (> 8.0)

http://chromforum.org/viewtopic.php?t=27307

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Best buffers to use at alkaline pH (> 8.0)

Posted: Sat Aug 29, 2015 2:39 pm
by saipraveen7
Dear all,
Could anyone please suggest me good buffers to use at alkaline pH (> 8.0)

Re: Best buffers to use at alkaline pH (> 8.0)

Posted: Sat Aug 29, 2015 6:35 pm
by tom jupille
http://www.hplc.eu/Downloads/ACE_Guide_ ... ection.pdf

Re: Best buffers to use at alkaline pH (> 8.0)

Posted: Sun Aug 30, 2015 1:21 pm
by mattmullaney
Hi Saipraveen,

Excellent reference from Tom Jupille--of the basic buffers listed within I've used borate, tris and glycine as parts of mobile phases in QC work.

Re: Best buffers to use at alkaline pH (> 8.0)

Posted: Mon Aug 31, 2015 11:13 am
by Gerhard Kratz
Ammonia, pKa 9.2, pH range 8.2 - 10.2, volatile, <10mM and <30°C is recommended with MS use

Re: Best buffers to use at alkaline pH (> 8.0)

Posted: Fri Nov 16, 2018 3:51 am
by danielcmaia
This link has also a number of biological buffers that are suitable at alkaline pH, such as Tricine, Bicine and Ches.

http://www.hopaxfc.com/en/blog/useful-p ... al-buffers
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