siRNA separation

[weeker2001]

the duplex of siRNA
my iclass
column temperature 25
TEAA 100mM
mobile phase A
mobile phase B 20% A in ACN
gradient elution 35% --85%

[weeker2001]

anyone got the experience of oligonucleotide analysis ?

[Andy Alpert]

Typically one analyzes single-stranded oligonucleotides smaller than ~ 18 bases by reversed phase and by anion-exchange if they're larger. Anion-exchange is the method to use for double-stranded oligos. What exactly are you trying to separate from what?