alpha, Rs and column length
Posted: Mon Aug 14, 2006 10:23 am
by GR
Hi chromatographers!
I would like to calculate alpha and Rs for 250mm column length from measured values on the same 150 mm column. Is it possible and how?
Thanks!
Posted: Mon Aug 14, 2006 12:59 pm
by DR
You need to do some basic reading on chromatographic theory.
Try these:
http://www.shu.ac.uk/schools/sci/chem/t ... chrom1.htm
http://www.lcresources.com/resources/getstart/
http://www.forumsci.co.il/HPLC/program.html
If you read them correctly, you should find that, other things being equal, alpha should be independent of column length and that R should be a function of plate numbers (N). If you figure out how many plates you have based on your R for the 150mm column, calculating the R for 250mm should be pretty easy.
I've provided a couple of eggs, I'll leave the omlette making to you.