Chromatography Forum

i'm really right now,i have to compare the rf value that i got from my assignment with standard of rf value. I've already search the related links for standard of rf value and have no idea until now...Please help Me...
My assignment is analyzing the amino acid in the cereal, and i've already get the rf value of cereal by using paper cromatography but until now i can't find the standard of rf value..thx 4 helping...

You have to find the standard Rf of your cereal experimentally. Did you run a sample of the cereal when you ran your amino acid? If you did not, then your time was wasted.

Rf values will depend upon the mobile phase you used and the temperature of the system as well as the nature of your paper you used to spot your samples.

Spotting samples of the cereal spiked with your analyte of interest at different levels and spotting samples of the amino acid at different levels would be the technique used to estimate the content of the amino acid in your cereal.

I assumed you digested your cereal with 6N HCl to hydrolyze any cereal protein to the free amino acid HCl salt form.

Please clarify your question with more details if my answer has not been helpful.

best wishes,

Rod

thank u very much rod...i've already got my rf value more accurate right now...