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DEA in Chiral Separations

Posted: Wed Aug 02, 2017 1:42 pm
by Mike H.
Simple question. Is there a optimal DEA concentration above which the peak begins to front? ie. at 0.1% the tailing is 1.1 and at 0.2% the tailing is 0.8.

If not what would cause a peak to front, other than overloading. (I'm only working at 2mg/ml). thx