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Peak fronting in NP chiral chromatography

http://chromforum.org/viewtopic.php?t=47754

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Peak fronting in NP chiral chromatography

Posted: Tue Aug 08, 2017 4:07 pm
by Mike H.
I've noticed that my peaks front at 40°, but are symmetrical at 30°. This is a NP isocratic method 80:20:0.1 Heptane:EtOH:DEA. The causes of fronting that I'm aware of are sample overload, solvent mismatch, low solubility of sample in MP, but I never read that temp was a cause. Can somebody explain the mechanism? thx

Re: Peak fronting in NP chiral chromatography

Posted: Tue Aug 08, 2017 5:27 pm
by Rndirk
What kind of compounds are you analyzing? Do they all show the same effect?

Is it possible that the compounds show isomerization at elevated temperatures?
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