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96-Well Plates
Posted: Sun Feb 10, 2008 6:50 pm
by Stryder08
I am running a Waters Acquity UPLC with an LCT Premier XE (TOF). I have tried to use well plates in the past, but have always picked up contaminants from the cover films we have used. I really don't want to use aluminum foil, but if I have to, then I will.
Anyone ever use well plates? If so, what type of cover film do you use? We cannot use any adhesive-based film as the adhesive builds up on the needle. I have also tried using a pressue type seal (which is preferable), but had some contaminants due to the high sensitivity of the MS.
Suggestions?
Posted: Tue Feb 12, 2008 2:45 am
by matthew
Agilent makes 96 well plates, with glass inserts and septa:
http://www.chem.agilent.com/ecommerce/p ... =5065-4402
Aluminum foil will work fine if you aren't storing the plates for a long period of time.
Posted: Wed Feb 13, 2008 12:07 am
by JJG
I have used the plate mats from Sun Sri quite a bit, though they aren't exactly cheap. Have worked well for me.
http://www.sun-sri.com/life_science/micromat.aspx
Posted: Mon Mar 10, 2008 7:29 pm
by Roxanne42
We use Symyx plates, where the glass vials are covered with a teflon sheet, followed by one or two black rubber sheets (to seal) and then a metallic plate - all screwed down.
The teflon sheet is the only thing we have seen that we ensure that reactions don't get contaminated (lots of organic solvents are used!) - but we do run into the problem that once the sheet is pierced, some evaporation can occur through the aperture (especially when heating) and this can sometimes be a problem in analysis.
Haven't tried the SunSRI micromats yet (teflon-coated silicone and apparently resealable) so let me know if you do. They aren't cheap though (and neither are Agilent's little full plates!) - at least definitely not from an educational facility standpoint|!
Posted: Tue Mar 11, 2008 9:08 pm
by brewer