Low sensitivy of QQQ

[haiedc]

Hi all,

I am a new LC/MS user (several years experience on GC/MS though). Now I am having the prolem of sensitivity. Could you please give me some advice that I could increase the sensitivity : the tune, the modes, sourse etc..
Thanks for any suggestions

[Kostas Petritis]

What is the nature of the compounds of interest and what are you using as mobile phase? What brand is your LC/MS?

[sindhu]

The tuning parameters, modes, sources changes from molecule to molecule. Can you please inform me what is the molecule you are going to work and its mobile phase then probably I can help you out.

Also please note LC-MS ( Single quad system ) systems are not considered as high sensitive systems when compared to triple quads & ToF systems. Where as Ion Traps are very high sensitive systems.