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- Posts: 5
- Joined: Tue Jul 01, 2008 5:23 pm
As the title, I tried to analyze farnesyl pyrophosphate, mevalonate acid, geranyl diphosphate using LC/MS/MS. I met a lot of problems in the analysis.
1. the standards can hardly eluent from column under normal condition only after NH4HCO3 (pH 8.5) modifier added. Do anybody have idea what's the use for NH4HCO3?
2. when i tried to quantitate the farnesyl pyrophosphate, I found the signal decreasing when the concentration is lower around 2nM, while it works fine when the concentration is around 20nM. at first, I thought it maybe result from absorption on glass vials. After I compared with glass, plastic, de-activated glass, I can not say that. Do anyone have any idea about that?
Thank you very much!
1. the standards can hardly eluent from column under normal condition only after NH4HCO3 (pH 8.5) modifier added. Do anybody have idea what's the use for NH4HCO3?
2. when i tried to quantitate the farnesyl pyrophosphate, I found the signal decreasing when the concentration is lower around 2nM, while it works fine when the concentration is around 20nM. at first, I thought it maybe result from absorption on glass vials. After I compared with glass, plastic, de-activated glass, I can not say that. Do anyone have any idea about that?
Thank you very much!
